6UXE

Structure of the human mitochondrial desulfurase complex Nfs1-ISCU2(M140I)-ISD11 with E.coli ACP1 at 1.57 A resolution showing flexibility of N terminal end of ISCU2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-07-10
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.987
Spacegroup name	P 41 21 2
Unit cell lengths	86.410, 86.410, 245.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.980 - 1.570
R-factor	0.1576
Rwork	0.156
R-free	0.17960
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5usr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.980	1.626
High resolution limit [Å]	1.570	1.570
Number of reflections	130413	12829
<I/σ(I)>	19.59	2.14
Completeness [%]	100.0	99.9
Redundancy	14.5	14.5
CC(1/2)	0.999	0.725

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		288	0.1 M MES pH 6.0-6.5, 22% PEG400