6UWH
Intermediate engineered variant of I-OnuI meganuclease with improved thermostability and partially altered specificity
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 HF |
Temperature [K] | 108 |
Detector technology | CCD |
Collection date | 2019-05-17 |
Detector | RIGAKU SATURN 944+ |
Wavelength(s) | 1.5418 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.890, 72.985, 163.574 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.228 - 2.299 |
R-factor | 0.2082 |
Rwork | 0.204 |
R-free | 0.25020 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3qqy |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER (2.8.1) |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.380 |
High resolution limit [Å] | 2.299 | 4.950 | 2.300 |
Rmerge | 0.058 | 0.030 | 0.428 |
Rmeas | 0.061 | 0.032 | 0.461 |
Rpim | 0.020 | 0.009 | 0.166 |
Number of reflections | 21003 | 2326 | 2053 |
<I/σ(I)> | 12.9 | ||
Completeness [%] | 97.4 | 99.1 | 99.3 |
Redundancy | 8.6 | 11.5 | 7.2 |
CC(1/2) | 1.000 | 0.908 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 100mM sodium acetate pH 6.0, 200mM calcium acetate, 35% PEG 400 |