6URQ
Complex structure of human poly-ADP-ribosyltransferase TNKS1 ARC2-ARC3 and P antigen family member 4 (PAGE4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-04-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 134.908, 101.035, 75.418 |
Unit cell angles | 90.00, 107.23, 90.00 |
Refinement procedure
Resolution | 39.750 - 2.050 |
R-factor | 0.2062 |
Rwork | 0.204 |
R-free | 0.24630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3utm |
RMSD bond length | 0.007 |
RMSD bond angle | 0.822 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER ((1.14_3260: ???)) |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 39.750 | 2.100 |
High resolution limit [Å] | 2.050 | 2.050 |
Rmeas | 0.085 | 0.834 |
Rpim | 0.043 | 0.422 |
Number of reflections | 60143 | 4432 |
<I/σ(I)> | 10.2 | 2.1 |
Completeness [%] | 99.4 | 98.7 |
Redundancy | 3.8 | 3.8 |
CC(1/2) | 0.996 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 6 | 298 | 100 mM Ammonium Sulfate, 10 mM DTT, 100 mM MES pH 6.0, 7% w/v PEG4000, 1% 1,6-Hexanediol |