6URH
Crystal structure of broadly neutralizing antibody AR3X in complex with Hepatitis C virus envelope glycoprotein E2 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-19 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 64.730, 69.231, 176.699 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 64.730 | 2.270 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.112 | 1.400 |
Rmeas | 0.123 | 1.537 |
Rpim | 0.050 | 0.626 |
Total number of observations | 243765 | 20579 |
Number of reflections | 40956 | 3516 |
<I/σ(I)> | 9 | 1.4 |
Completeness [%] | 99.4 | 99.6 |
Redundancy | 6 | 5.9 |
CC(1/2) | 0.993 | 0.375 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 0.25 M ammonium tartrate dibasic pH 7.0, 20% PEG 3350 |