6UPR
Crystal Structure of GTPase Domain of Human Septin 2 / Septin 8 Heterocomplex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I04 |
Synchrotron site | Diamond |
Beamline | I04 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-07-06 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 52.754, 82.773, 78.144 |
Unit cell angles | 90.00, 103.63, 90.00 |
Refinement procedure
Resolution | 55.959 - 2.299 |
R-factor | 0.2105 |
Rwork | 0.208 |
R-free | 0.25470 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6upq |
Data reduction software | xia2 |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 82.800 | 82.800 | 2.360 |
High resolution limit [Å] | 2.299 | 10.290 | 2.300 |
Rmerge | 0.131 | 0.047 | 1.688 |
Rmeas | 0.142 | 0.052 | 1.821 |
Rpim | 0.054 | 0.021 | 0.680 |
Total number of observations | 2161 | 14951 | |
Number of reflections | 28853 | 348 | 2108 |
<I/σ(I)> | 8 | 34.6 | 1 |
Completeness [%] | 99.2 | 99.5 | 99.5 |
Redundancy | 6.9 | 6.2 | 7.1 |
CC(1/2) | 0.992 | 0.994 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 291 | 100mM MES/imidazole pH 6.5, 10% PEG 8000, 20% ethylene glycol and 20mM of each 1,6-hexanediol, 1-butanol, (RS)-1,2-propanediol, 2-propanol, 1,4-butanediol and 1,3-propanediol |