6UOL
Y271G DNA polymerase beta substrate complex with a templating cytosine and incoming rGTP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-05 |
| Detector | DECTRIS PILATUS3 R 200K-A |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.800, 80.400, 55.500 |
| Unit cell angles | 90.00, 107.90, 90.00 |
Refinement procedure
| Resolution | 25.088 - 1.936 |
| R-factor | 0.1927 |
| Rwork | 0.186 |
| R-free | 0.24960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2fms |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.088 | 25.000 | 1.980 |
| High resolution limit [Å] | 1.936 | 5.280 | 1.950 |
| Rmerge | 0.042 | 0.023 | 0.864 |
| Rmeas | 0.047 | 0.026 | |
| Rpim | 0.022 | 0.011 | 0.677 |
| Number of reflections | 42206 | 1609 | 1498 |
| <I/σ(I)> | 14.3 | ||
| Completeness [%] | 99.3 | 100 | 98.2 |
| Redundancy | 4 | 5.5 | 2.1 |
| CC(1/2) | 0.999 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 19% PEG3350, 0.35 M sodium acetate, 0.05 M Imidazole, pH 8.0. |
