6UO1
Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA (containing pseudouridine at the first position of the codon) and deacylated A-, P-, and E-site tRNAs at 2.95A resolution
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-02 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.97920 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 208.730, 445.100, 613.630 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 360.297 - 2.950 |
R-factor | 0.241 |
Rwork | 0.238 |
R-free | 0.29240 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4y4p |
RMSD bond length | 0.003 |
RMSD bond angle | 0.650 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.8.2) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 360.297 | 360.297 | 3.030 |
High resolution limit [Å] | 2.950 | 13.190 | 2.950 |
Rmerge | 0.206 | 0.111 | 1.798 |
Rmeas | 0.228 | 0.121 | 2.016 |
Total number of observations | 5954867 | ||
Number of reflections | 1181400 | 13883 | 86854 |
<I/σ(I)> | 4.95 | 17.5 | 0.89 |
Completeness [%] | 99.7 | 98.5 | 99.6 |
Redundancy | 5.041 | 5.856 | 4.765 |
CC(1/2) | 0.995 | 0.995 | 0.172 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.6 | 292 | 0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME |