6UO1

Crystal structure of the Thermus thermophilus 70S ribosome in complex with mRNA (containing pseudouridine at the first position of the codon) and deacylated A-, P-, and E-site tRNAs at 2.95A resolution

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-02
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.97920
Spacegroup name	P 21 21 21
Unit cell lengths	208.730, 445.100, 613.630
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	360.297 - 2.950
R-factor	0.241
Rwork	0.238
R-free	0.29240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4y4p
RMSD bond length	0.003
RMSD bond angle	0.650
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.8.2)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	360.297	360.297	3.030
High resolution limit [Å]	2.950	13.190	2.950
Rmerge	0.206	0.111	1.798
Rmeas	0.228	0.121	2.016
Total number of observations	5954867
Number of reflections	1181400	13883	86854
<I/σ(I)>	4.95	17.5	0.89
Completeness [%]	99.7	98.5	99.6
Redundancy	5.041	5.856	4.765
CC(1/2)	0.995	0.995	0.172

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.6	292	0.1-0.2 M Arginine-HCl, 0.1M Tris-HCl pH 7.6, 2.5% PEG-20K, 7-12% MPD, 0.5 mM BME