6UI2
Structure of human DNA polymerase eta complexed with N7MG in the template base paired with incoming non-hydrolyzable CTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.3 |
Synchrotron site | ALS |
Beamline | 5.0.3 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-07-26 |
Detector | ADSC QUANTUM 315 |
Wavelength(s) | 0.97648 |
Spacegroup name | P 61 |
Unit cell lengths | 98.684, 98.684, 81.852 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.900 - 2.350 |
R-factor | 0.1738 |
Rwork | 0.170 |
R-free | 0.23740 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4o3n |
RMSD bond length | 0.009 |
RMSD bond angle | 1.565 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 20.000 | 20.000 | 2.390 |
High resolution limit [Å] | 2.339 | 6.310 | 2.350 |
Rmerge | 0.142 | 0.050 | 0.454 |
Number of reflections | 19191 | 997 | 942 |
<I/σ(I)> | 6.1 | ||
Completeness [%] | 100.0 | 100 | 100 |
Redundancy | 11.3 | 11.2 | 11.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 0.1M MES (pH 5.5), 5 mM MgCl2, and 15 to 20 % PEG 2K-MME |