6UI2

Structure of human DNA polymerase eta complexed with N7MG in the template base paired with incoming non-hydrolyzable CTP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.3
Synchrotron site	ALS
Beamline	5.0.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-26
Detector	ADSC QUANTUM 315
Wavelength(s)	0.97648
Spacegroup name	P 61
Unit cell lengths	98.684, 98.684, 81.852
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.900 - 2.350
R-factor	0.1738
Rwork	0.170
R-free	0.23740
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4o3n
RMSD bond length	0.009
RMSD bond angle	1.565
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	20.000	20.000	2.390
High resolution limit [Å]	2.339	6.310	2.350
Rmerge	0.142	0.050	0.454
Number of reflections	19191	997	942
<I/σ(I)>	6.1
Completeness [%]	100.0	100	100
Redundancy	11.3	11.2	11.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	0.1M MES (pH 5.5), 5 mM MgCl2, and 15 to 20 % PEG 2K-MME