6UE6
PWWP1 domain of NSD2 in complex with MR837
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-09-17 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 69.210, 70.320, 228.830 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.300 - 2.400 |
R-factor | 0.236 |
Rwork | 0.235 |
R-free | 0.25200 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vc8 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.330 | 49.330 | 2.490 |
High resolution limit [Å] | 2.400 | 8.980 | 2.400 |
Rmerge | 0.084 | 0.043 | 0.997 |
Rmeas | 0.092 | 0.048 | 1.084 |
Number of reflections | 44525 | 974 | 4500 |
<I/σ(I)> | 14 | 36.9 | 1.9 |
Completeness [%] | 99.7 | ||
Redundancy | 6.4 | 5.2 | 6.5 |
CC(1/2) | 0.998 | 0.997 | 0.729 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 291 | 20% PEG3350, 0.2 M potassium thiocyanate |