6U9K
MLL1 SET N3861I/Q3867L bound to inhibitor 18 (TC-5153)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-02-07 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.9786 |
Spacegroup name | P 32 |
Unit cell lengths | 54.949, 54.949, 104.895 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.340 - 2.000 |
R-factor | 0.209 |
Rwork | 0.207 |
R-free | 0.24400 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5f5e |
RMSD bond length | 0.010 |
RMSD bond angle | 1.020 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.030 |
High resolution limit [Å] | 2.000 | 2.000 |
Number of reflections | 23928 | 23928 |
<I/σ(I)> | 10 | 2 |
Completeness [%] | 100.0 | |
Redundancy | 7.8 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 2.2 M Sodium Malonate pH 7.0 |