6U8W
Crystal structure of DNMT3B(K777A)-DNMT3L in complex with CpGpT DNA
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.2 |
Synchrotron site | ALS |
Beamline | 5.0.2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-06-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9774 |
Spacegroup name | P 31 |
Unit cell lengths | 193.506, 193.506, 49.869 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 48.377 - 2.949 |
R-factor | 0.216714672433 |
Rwork | 0.216 |
R-free | 0.23632 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5yx2 |
RMSD bond length | 0.004 |
RMSD bond angle | 0.685 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.12_2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.380 | 3.050 |
High resolution limit [Å] | 2.949 | 2.950 |
Rmerge | 0.097 | 0.733 |
Number of reflections | 43944 | 4333 |
<I/σ(I)> | 7.91 | 1.34 |
Completeness [%] | 99.7 | |
Redundancy | 3.4 | 3.3 |
CC(1/2) | 0.997 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8 | 277.15 | 0.1 M Tris-HCl (pH 8.0), 200 mM MgCl2, 8% PEG 4000 |