6U7C

Human GRK2 in complex with Gbetagamma subunits and CCG258747

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	CCD
Collection date	2016-11-10
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0332
Spacegroup name	C 2 2 21
Unit cell lengths	60.633, 240.610, 214.576
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.902 - 2.440
R-factor	0.2375
Rwork	0.237
R-free	0.26240
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4pnk
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.920	2.527
High resolution limit [Å]	2.440	2.440
Rmerge	0.237	3.939
Rmeas	0.252
Rpim	0.068	1.592
Number of reflections	112767	5845
<I/σ(I)>	7.25	0.47
Completeness [%]	99.2	95.66
Redundancy	13.5
CC(1/2)	0.997	0.405

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	277	50 mM MES pH 6.0, 1.1 M NaCl, 6% PEG 3350