6U39
2.4 Angstrom crystal structure of the D129G Ca-CaM:CaV1.2 IQ domain complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-27 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 121.795, 161.656, 96.277 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.041 - 2.400 |
R-factor | 0.2344 |
Rwork | 0.232 |
R-free | 0.28270 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3g43 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 49.041 | 49.041 | 2.440 |
High resolution limit [Å] | 2.300 | 6.840 | 2.300 |
Number of reflections | 159034 | 6041 | 25711 |
<I/σ(I)> | 17.06 | ||
Completeness [%] | 97.5 | ||
Redundancy | 2.93 | ||
CC(1/2) | 0.999 | 0.998 | 0.799 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | ammonium sulfate |