6U2H

BRAF dimer bound to 14-3-3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	77
Detector technology	PIXEL
Collection date	2019-07-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	61.504, 115.752, 153.092
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.454 - 2.500
R-factor	0.2215
Rwork	0.220
R-free	0.26320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4mnf 5nas
RMSD bond length	0.011
RMSD bond angle	1.345
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.12-2829_final)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	34.454	2.589
High resolution limit [Å]	2.500	2.500
Rmerge	0.081	1.718
Number of reflections	72587	26121
<I/σ(I)>	13.91	0.88
Completeness [%]	99.3	99.27
Redundancy	6.7
CC(1/2)	0.999	0.528

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	291	PEG 6000 Tris pH 7.5