6TYY
Hedgehog autoprocessing mutant D46H
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL14-1 |
Synchrotron site | SSRL |
Beamline | BL14-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-06-15 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.1271 |
Spacegroup name | P 61 |
Unit cell lengths | 78.381, 78.381, 37.351 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 25.656 - 1.360 |
R-factor | 0.1722 |
Rwork | 0.171 |
R-free | 0.19490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1at0 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHENIX |
Refinement software | PHENIX |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 100.000 | 100.000 | 1.380 |
High resolution limit [Å] | 1.360 | 3.690 | 1.360 |
Rmerge | 0.058 | 0.029 | 0.924 |
Rmeas | 0.066 | 0.033 | 1.134 |
Rpim | 0.030 | 0.015 | 0.642 |
Total number of observations | 131992 | ||
Number of reflections | 28219 | 1472 | 1367 |
<I/σ(I)> | 11 | ||
Completeness [%] | 99.6 | 98.8 | 96.3 |
Redundancy | 4.7 | 4.6 | 2.9 |
CC(1/2) | 0.999 | 0.624 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 0.1 M Hepes, pH 7.5, 3.4 M sodium formate |