6TQS
The crystal structure of the MSP domain of human MOSPD2 in complex with the conventional FFAT motif of ORP1.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-25 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.98 |
Spacegroup name | P 61 2 2 |
Unit cell lengths | 126.874, 126.874, 184.449 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 70.640 - 2.250 |
R-factor | 0.219 |
Rwork | 0.217 |
R-free | 0.26000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wic |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER (2.8.2) |
Refinement software | PHENIX (dev-3644) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 109.880 | 109.880 | 2.320 |
High resolution limit [Å] | 2.250 | 9.000 | 2.250 |
Rmerge | 0.244 | 0.071 | 5.314 |
Rmeas | 0.248 | 0.072 | 5.413 |
Rpim | 0.040 | 0.013 | 1.023 |
Total number of observations | 1549132 | 25996 | 105858 |
Number of reflections | 42297 | 803 | 3815 |
<I/σ(I)> | 11.3 | 34.4 | 0.7 |
Completeness [%] | 100.0 | 100 | 99.9 |
Redundancy | 36.6 | 32.4 | 27.7 |
CC(1/2) | 0.999 | 1.000 | 0.507 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 20% PEG 3350, 0.2 M sodium sulfate |