6TL8
Structural basis of SALM3 dimerization and adhesion complex formation with the presynaptic receptor protein tyrosine phosphatases
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-05-04 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97625 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 31.450, 132.160, 134.180 |
Unit cell angles | 90.00, 90.08, 90.00 |
Refinement procedure
Resolution | 30.000 - 2.800 |
R-factor | 0.2631 |
Rwork | 0.262 |
R-free | 0.28500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | PDB 6F2O |
RMSD bond length | 0.006 |
RMSD bond angle | 0.880 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.870 |
High resolution limit [Å] | 2.790 | 2.790 |
Rmerge | 0.170 | 1.190 |
Number of reflections | 26991 | 1886 |
<I/σ(I)> | 9 | 2.1 |
Completeness [%] | 99.2 | |
Redundancy | 5.9 | |
CC(1/2) | 0.995 | 0.700 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 7.5 | 293 | 0.17 M ammonium sulfate pH 7.5, 25% w/v PEG 4000, 10% glycerol |