6TBL

Crystal structure of MMS19(CTD)-CIAO1-CIAO2B CIA targeting complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	292
Detector technology	PIXEL
Collection date	2018-04-15
Detector	DECTRIS PILATUS 2M
Wavelength(s)	0.999995
Spacegroup name	P 4 21 2
Unit cell lengths	144.070, 144.070, 166.430
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 2.650
R-factor	0.1999
Rwork	0.198
R-free	0.24220
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	CIAO1-CIAO2B structure to be deposited
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.990	2.744
High resolution limit [Å]	2.650	2.650
Rmerge	0.212
Rmeas	0.215
Rpim	0.029
Number of reflections	51309	5018
<I/σ(I)>	23.53	0.83
Completeness [%]	99.6	99.98
Redundancy	53.1	50.4
CC(1/2)	1.000	0.363

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.2 M KSCN, 20% PEG 3350