6SXF
Crystal Structure of the Voltage-Gated Sodium Channel NavMs (F208L) in complex with Tamoxifen (2.8 Angstrom resolution)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-02-15 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9686 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 107.956, 107.956, 212.224 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 76.340 - 2.839 |
R-factor | 0.2222 |
Rwork | 0.220 |
R-free | 0.26850 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5hvx |
RMSD bond length | 0.008 |
RMSD bond angle | 0.890 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3 (19-MAR-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 76.340 | 2.910 |
High resolution limit [Å] | 2.839 | 2.840 |
Number of reflections | 30492 | 2195 |
<I/σ(I)> | 6.3 | 1.2 |
Completeness [%] | 100.0 | 100 |
Redundancy | 15.4 | 15.8 |
CC(1/2) | 0.980 | 0.865 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 277 | 0.05 M NaCl, 0.1 M MES buffer, pH 6.5, 37.8% v/v polyethylene glycol (PEG) 400 and 4% ethylene glycol |