6ST2
Selective Affimers Recognize BCL-2 Family Proteins Through Non-Canonical Structural Motifs
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-12-10 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 71.891, 71.891, 204.057 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 102.100 | 1.840 |
High resolution limit [Å] | 1.790 | 1.790 |
Rmerge | 0.079 | 4.694 |
Rmeas | 0.085 | 5.044 |
Rpim | 0.031 | 1.835 |
Number of reflections | 51639 | 3727 |
<I/σ(I)> | 13.8 | 1.1 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.9 | 14.2 |
CC(1/2) | 0.990 | 0.530 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 10% PEG 8000, 0.2M magnesium chloride and 0.1M Tris pH 7.0 |