6SSP
Structure of the pentameric ligand-gated ion channel ELIC in complex with a NAM nanobody
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2011-11-26 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 1 |
| Unit cell lengths | 121.804, 122.354, 128.221 |
| Unit cell angles | 71.02, 64.19, 61.66 |
Refinement procedure
| Resolution | 48.320 - 3.250 |
| R-factor | 0.249 |
| Rwork | 0.248 |
| R-free | 0.26400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vl0 3p0g |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS (Mar 15, 2019) |
| Data scaling software | Aimless (0.7.4) |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.16-3549) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.320 | 48.320 | 3.420 |
| High resolution limit [Å] | 3.250 | 10.270 | 3.250 |
| Rmerge | 0.053 | 0.017 | 0.807 |
| Rmeas | 0.074 | 0.024 | 1.141 |
| Rpim | 0.053 | 0.017 | 0.807 |
| Total number of observations | 146197 | 5357 | 13600 |
| Number of reflections | 82177 | 2812 | 8045 |
| <I/σ(I)> | 5.6 | 18.5 | 0.7 |
| Completeness [%] | 89.8 | 97.3 | 60.3 |
| Redundancy | 1.8 | 1.9 | 1.7 |
| CC(1/2) | 0.993 | 0.991 | 0.483 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 293 | 0.1 M Na2SO4, 0.1 M bis-trispropane, 10% PEG3350 |
