6SSH

Structure of the TSC2 GAP domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.3
Synchrotron site	BESSY
Beamline	14.3
Temperature [K]	100
Detector technology	CCD
Collection date	2015-06-04
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.89429
Spacegroup name	P 21 21 21
Unit cell lengths	53.895, 58.329, 70.700
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.000 - 1.400
Rwork	0.160
R-free	0.18560
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1sqr
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHENIX
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.000	1.480
High resolution limit [Å]	1.400	1.400
Rmeas	0.061	0.761
Number of reflections	44616	7070
<I/σ(I)>	20.85	2.25
Completeness [%]	99.9
Redundancy	6.93

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293.15	0.1 M CHES pH 9.5, 20 % PEG 8000