6SQQ
Structure of the U1A variant A1-98 Y31H/Q36R/F56W triple mutant in complex with RNA obtained by soaking
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-01 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.03320 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 96.080, 96.080, 258.010 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 51.031 - 2.370 |
Rwork | 0.245 |
R-free | 0.29410 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1urn |
RMSD bond length | 0.009 |
RMSD bond angle | 1.592 |
Data reduction software | xia2 |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 51.600 | 2.410 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.290 | 2.601 |
Rmeas | 0.293 | 2.633 |
Number of reflections | 29657 | 1460 |
<I/σ(I)> | 12.4 | 1.4 |
Completeness [%] | 100.0 | 99.9 |
Redundancy | 38.2 | 40.2 |
CC(1/2) | 0.998 | 0.753 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 2.2 M ammonium sulfate, 0.2 M tri-potassium citrate |