1URN

U1A MUTANT/RNA COMPLEX + GLYCEROL

Summary for 1URN

• Entry DOI: 10.2210/pdb1urn/pdb
• Descriptor: RNA (5'-R(*AP*AP*UP*CP*CP*AP*UP*UP*GP*CP*AP*CP*UP*CP*CP*GP*G P*AP*UP*UP*U)-3'), PROTEIN (U1A), CHLORIDE ION, ... (5 entities in total)
• Functional Keywords: protein-rna complex, single strand, overhanging base, hairpin loop, transcription-rna complex, transcription/rna
• Biological source: Homo sapiens (human)
• Cellular location: Nucleus: P09012
• Total number of polymer chains: 6
• Total formula weight: 53864.01

Authors

Oubridge, C.,Ito, N.,Evans, P.R.,Teo, C.-H.,Nagai, K. (deposition date: 1995-01-04, release date: 1996-03-08, Last modification date: 2024-02-14)

Primary citation

Oubridge, C.,Ito, N.,Evans, P.R.,Teo, C.H.,Nagai, K.
Crystal structure at 1.92 A resolution of the RNA-binding domain of the U1A spliceosomal protein complexed with an RNA hairpin.
Nature, 372:432-438, 1994

PubMed Abstract: The crystal structure of the RNA-binding domain of the small nuclear ribonucleoprotein U1A bound to a 21-nucleotide RNA hairpin has been determined at 1.92 A resolution. The ten-nucleotide RNA loop binds to the surface of the beta-sheet as an open structure, and the AUUGCAC sequence of the loop interacts extensively with the conserved RNP1 and RNP2 motifs and the C-terminal extension of the RNP domain. These interactions include stacking of RNA bases with aromatic side chains of proteins and many direct and water-mediated hydrogen bonds. The structure reveals the stereochemical basis for sequence-specific RNA recognition by the RNP domain.

PubMed: 7984237
DOI: 10.1038/372432a0

Experimental method

X-RAY DIFFRACTION (1.92 Å)