6SN1
Crystal structure of the human INTS13-INTS14 complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X06DA |
Synchrotron site | SLS |
Beamline | X06DA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-11-11 |
Detector | DECTRIS PILATUS 2M-F |
Wavelength(s) | 1.0000 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 190.987, 115.354, 147.819 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 45.470 - 2.540 |
R-factor | 0.2252 |
Rwork | 0.224 |
R-free | 0.24370 |
Structure solution method | MAD |
RMSD bond length | 0.002 |
RMSD bond angle | 0.481 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | MLPHARE |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.470 | 2.630 |
High resolution limit [Å] | 2.540 | 2.540 |
Rmeas | 0.149 | 3.806 |
Rpim | 0.040 | 1.012 |
Number of reflections | 107306 | 10162 |
<I/σ(I)> | 16.2 | 0.6 |
Completeness [%] | 99.5 | 95.33 |
Redundancy | 13.7 | |
CC(1/2) | 0.999 | 0.354 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 291 | 0.1 M Bis-Tris (pH 6.0), 0.75 M AmSO4 and 1% (w/v) PEG 3350 |