6SIV

Structure of HPV16 E6 oncoprotein in complex with mutant IRF3 LxxLL motif

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-04-27
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1
Spacegroup name	P 21 21 2
Unit cell lengths	97.031, 132.467, 42.634
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.516 - 1.752
Rwork	0.182
R-free	0.22020
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.16rc1_3531)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.516	1.860
High resolution limit [Å]	1.752	1.752
Number of reflections	104672	16656
<I/σ(I)>	13.54
Completeness [%]	97.9
Redundancy	12.2
CC(1/2)	0.999	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION		298	PEG 1500 30%