6SH6

Crystal structure of the human DEAH-helicase DHX15 in complex with the NKRF G-patch bound to ADP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-05-22
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	0.999999
Spacegroup name	C 2 2 21
Unit cell lengths	82.855, 91.212, 212.839
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.400 - 1.850
R-factor	0.2055
Rwork	0.203
R-free	0.24430
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5xdr
RMSD bond length	0.010
RMSD bond angle	1.042
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.400	1.920
High resolution limit [Å]	1.850	1.850
Rmeas	0.148	4.220
Rpim	0.040	1.130
Number of reflections	68754	6760
<I/σ(I)>	13.4	0.7
Completeness [%]	99.9	98.9
Redundancy	13.4	13.6
CC(1/2)	0.999	0.387

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	291.15	0.02 M 1,6-hexanediol, 0.02 M 1-butanol, 0.02 M (RS)-1,2-propanediol, 0.02 M 2-propanol, 0.02 M 1,4-butanediol, 0.02 M 1,3-propanediol, 0.1 M MES/imidazole pH 6.5, 10% w/v PEG 8000, 20% v/v ethylene glycol