6SH2
Crystal structure of human neprilysin E584D in complex with C-type natriuretic peptide.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I03 |
Synchrotron site | Diamond |
Beamline | I03 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-13 |
Detector | DECTRIS EIGER2 X 16M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 32 2 1 |
Unit cell lengths | 109.267, 109.267, 112.579 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 72.440 - 2.600 |
Rwork | 0.243 |
R-free | 0.28660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6gid |
RMSD bond length | 0.002 |
RMSD bond angle | 1.148 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 72.440 | 2.720 |
High resolution limit [Å] | 2.600 | 2.600 |
Number of reflections | 24386 | 2945 |
<I/σ(I)> | 17.3 | |
Completeness [%] | 100.0 | |
Redundancy | 39.9 | |
CC(1/2) | 1.000 | 0.604 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 291.15 | Potassium nitrate, Sodium iodide, PEG 3350 |