6SF1

Bone morphogenetic protein 10 (BMP10) complexed with extracellular domain of activin receptor-like kinase 1 (ALK1).

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-09-24
Detector	DECTRIS PILATUS 6M-F
Wavelength(s)	0.976
Spacegroup name	P 65 2 2
Unit cell lengths	57.570, 57.570, 304.062
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	24.930 - 2.800
R-factor	0.2477
Rwork	0.244
R-free	0.27890
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4fao
RMSD bond length	0.002
RMSD bond angle	0.488
Data reduction software	iMOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX (1.17_3644)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.030	2.950
High resolution limit [Å]	2.800	2.800
Rmerge	0.097	0.519
Rpim	0.052
Number of reflections	8187	1151
<I/σ(I)>	9.5	2.6
Completeness [%]	99.7	100
Redundancy	4.3	4.6
CC(1/2)	0.995	0.862

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	17% PEG 3350, 0.175 M NH4I, 0.1 M HEPES pH 7.8