6S9J
Crystal structure of TfR1 mimicry in complex with GP1 from MACV
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID30B |
| Synchrotron site | ESRF |
| Beamline | ID30B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-03-08 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.9198 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 104.626, 104.626, 281.363 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.559 - 2.698 |
| R-factor | 0.2426 |
| Rwork | 0.241 |
| R-free | 0.26650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3kas |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.949 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((dev_2871: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.750 |
| High resolution limit [Å] | 2.698 | 2.698 |
| Rpim | 0.038 | 0.483 |
| Number of reflections | 41703 | 1000 |
| <I/σ(I)> | 17.2 | 0.9 |
| Completeness [%] | 94.3 | 46.5 |
| Redundancy | 10.2 | |
| CC(1/2) | 0.998 | 0.300 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.2 M Na Thiocyanate pH 6.9, 20% PEG 3350 and 5% MPD |
