6S8S
Extended structure of the human DDX6 C-terminal domain in complex with an EDC3 FDF peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 90 |
Detector technology | CCD |
Collection date | 2008-06-19 |
Detector | MARRESEARCH |
Wavelength(s) | 0.978 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 172.425, 47.878, 65.757 |
Unit cell angles | 90.00, 96.34, 90.00 |
Refinement procedure
Resolution | 25.310 - 2.210 |
R-factor | 0.181 |
Rwork | 0.179 |
R-free | 0.22600 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2wax |
RMSD bond length | 0.010 |
RMSD bond angle | 1.060 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.3) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 25.310 | 2.250 |
High resolution limit [Å] | 2.210 | 2.210 |
Number of reflections | 25958 | 961 |
<I/σ(I)> | 9.6 | |
Completeness [%] | 95.6 | |
Redundancy | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 292 | 0.1 M CAPS pH 10.5, 1.2 M NaH2PO4, 0.8 M K2HPO4, 0.2 M Li2SO4 |