Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2014-11-20 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.420, 169.082, 83.730 |
| Unit cell angles | 90.00, 92.81, 90.00 |
Refinement procedure
| Resolution | 83.630 - 3.500 |
| R-factor | 0.237 |
| Rwork | 0.235 |
| R-free | 0.27110 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6GT1 |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.482 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 83.630 | 3.720 |
| High resolution limit [Å] | 3.500 | 3.500 |
| Rmerge | 0.176 | |
| Number of reflections | 17292 | 1698 |
| <I/σ(I)> | 4.9 | |
| Completeness [%] | 99.1 | |
| Redundancy | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 291 | 0.02 M Sodium/potassium phosphate, 0.1 M Bis-Tris propane pH 6.5, 20 % w/v PEG 3350 |
