6S5V

Crystal structure of the Cap-Midlink region of the H5N1 Influenza A virus polymerase in complex with a Cap-domain binding analogue

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-09-08
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.9763
Spacegroup name	P 43 21 2
Unit cell lengths	105.703, 105.703, 140.657
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	84.500 - 1.350
R-factor	0.1487
Rwork	0.148
R-free	0.16480
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.069
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	84.500	1.400
High resolution limit [Å]	1.350	1.350
Rmerge	0.017	0.630
Rmeas	0.024	0.830
Rpim	0.017	0.630
Number of reflections	173842	17177
<I/σ(I)>	16.91	0.9
Completeness [%]	100.0	99.95
Redundancy	2	2
CC(1/2)	1.000	0.520

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.2	293	10 % PEG 3000, 0.2 M NaCl, and K-citrate, pH 4.2