6RZ7
Crystal structure of the human cysteinyl leukotriene receptor 2 in complex with ONO-2570366 (F222 space group)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID30B |
Synchrotron site | ESRF |
Beamline | ID30B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.07228 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 81.830, 142.070, 171.160 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 19.930 - 2.430 |
R-factor | 0.2309 |
Rwork | 0.229 |
R-free | 0.25870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6rz4 |
RMSD bond length | 0.007 |
RMSD bond angle | 1.110 |
Data reduction software | XDS (20161205) |
Data scaling software | XSCALE (20180319) |
Phasing software | PHASER (1.12-2829) |
Refinement software | PHENIX (1.12-2829) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.520 |
High resolution limit [Å] | 2.430 | 2.430 |
Rmerge | 0.194 | 2.168 |
Rpim | 0.108 | 1.192 |
Number of reflections | 17665 | |
<I/σ(I)> | 4.8 | 0.7 |
Completeness [%] | 92.4 | 89.2 |
Redundancy | 3.8 | 3.9 |
CC(1/2) | 0.990 | 0.220 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 7 | 293 | 30 mM NH4 tartrate dibasic 24% PEG400 100 mM HEPES 7.0 |