6RVY
Inward-open structure of the ASCT2 (SLC1A5) mutant C467R in absence of substrate
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TALOS ARCTICA |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 200 |
| Cs (mm) | 2.7 |
| Energy Filter | GIF Bioquantum |
| Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 4.13 |
| Number of Particle | 323403 |
| Software | RELION,EPU,CTFFIND,Coot,PHENIX |
| Symmetry | C3 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
