6RFU
In cellulo crystallization of Trypanosoma brucei IMP dehydrogenase enables the identification of ATP and GMP as genuine co-factors
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | FREE ELECTRON LASER |
Source details | SLAC LCLS BEAMLINE CXI |
Synchrotron site | SLAC LCLS |
Beamline | CXI |
Temperature [K] | 291 |
Detector technology | PIXEL |
Collection date | 2012-01-28 |
Detector | CS-PAD XPP |
Wavelength(s) | 2.07 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 209.000, 209.000, 92.000 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.300 - 2.800 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1jcn |
Data reduction software | CrystFEL |
Data scaling software | CrystFEL |
Phasing software | PHASER |
Refinement software | PHENIX |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.300 | 2.870 |
High resolution limit [Å] | 2.800 | 2.800 |
Number of reflections | 50656 | 3557 |
<I/σ(I)> | 4.14 | |
Completeness [%] | 100.0 | |
Redundancy | 187 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | IN CELL | 291 | In cellulo upon cell growth |