6RFO
ERK2 MAP kinase with the activation loop of p38alpha
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-02-05 |
Detector | DECTRIS PILATUS3 R CdTe 300K |
Wavelength(s) | 0.972 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 47.999, 68.688, 60.231 |
Unit cell angles | 90.00, 109.13, 90.00 |
Refinement procedure
Resolution | 45.390 - 1.700 |
R-factor | 0.15969 |
Rwork | 0.156 |
R-free | 0.21874 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4s31 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.894 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.390 | 1.730 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 40460 | 2160 |
<I/σ(I)> | 10 | |
Completeness [%] | 99.4 | |
Redundancy | 4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.8 M ammonium sulfate, 0.1 M Bis-tris pH 6.8 or 6.5, 2% (w/v) PEG 550 |