6RFK
Crystal structure of EGRCK-inhibited Gla-domainless fIXa (K148Q, R150Q variant)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-08-15 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 0.97949 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 100.160, 100.160, 91.500 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 70.820 - 1.600 |
| R-factor | 0.12792 |
| Rwork | 0.126 |
| R-free | 0.16314 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5jb8 |
| RMSD bond length | 0.033 |
| RMSD bond angle | 2.656 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0155) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 70.820 | 1.630 |
| High resolution limit [Å] | 1.600 | 1.600 |
| Number of reflections | 61775 | 2963 |
| <I/σ(I)> | 13.9 | |
| Completeness [%] | 99.9 | |
| Redundancy | 7.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 297 | 0.1M BIS-TRIS propane pH 8.5, 24% PEG 3350, 0.275M Na Malonate |
