6R75
Crystal structure of human Ube2T E54R mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE MASSIF-1 |
| Synchrotron site | ESRF |
| Beamline | MASSIF-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-06-14 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.966 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.130, 46.190, 74.200 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.210 - 2.000 |
| R-factor | 0.2271 |
| Rwork | 0.225 |
| R-free | 0.26440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1yh2 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.406 |
| Data reduction software | MOSFLM |
| Data scaling software | Aimless (0.7.3) |
| Phasing software | PHASER |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 46.190 | 46.190 | 2.050 |
| High resolution limit [Å] | 2.000 | 8.940 | 2.000 |
| Rmerge | 0.045 | 0.036 | 0.100 |
| Rmeas | 0.053 | 0.042 | 0.115 |
| Rpim | 0.028 | 0.021 | 0.056 |
| Total number of observations | 34140 | ||
| Number of reflections | 9878 | 137 | 769 |
| <I/σ(I)> | 16.7 | ||
| Completeness [%] | 88.7 | 87.5 | 94.6 |
| Redundancy | 3.5 | 3.5 | 3.6 |
| CC(1/2) | 0.997 | 0.999 | 0.988 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M Tris-HCl pH 8.5, 20% v/v glycerol ethoxylate, 3% v/v poly(ethylene imine) |
