6R4J

Crystal structure of human GFAT-1 G451E in complex with UDP-GlcNAc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-04-26
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.0
Spacegroup name	P 41 21 2
Unit cell lengths	153.227, 153.227, 162.544
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.727 - 2.420
R-factor	0.1949
Rwork	0.194
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6r4e
RMSD bond length	0.002
RMSD bond angle	0.444
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	XDS (Jan 26, 2018)
Phasing software	PHENIX ((dev_2499: ???))
Refinement software	PHENIX ((dev_2499: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	2.510
High resolution limit [Å]	2.420	2.420
Number of reflections	74011	7226
<I/σ(I)>	18.36
Completeness [%]	99.9
Redundancy	13.4
CC(1/2)	0.999	0.599

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Bis tris propane pH 9.0, 0.4 M Potassium sodium tartrate, 20 % PEG3350