6R0X

The extracellular domain of G6b-B in complex with Fab fragment and DP12 heparin oligosaccharide.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-03
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97624
Spacegroup name	C 1 2 1
Unit cell lengths	183.800, 72.340, 131.042
Unit cell angles	90.00, 124.52, 90.00

Refinement procedure

Resolution	63.100 - 3.130
R-factor	0.232
Rwork	0.230
R-free	0.26600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4k2u
Data reduction software	XDS (Jan 26, 2018)
Data scaling software	Aimless (0.7.1)
Phasing software	PHASER (2.8.2)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	65.270	65.270	3.300
High resolution limit [Å]	3.130	9.910	3.130
Rmerge	0.159	0.037	1.214
Rmeas	0.193	0.046	1.469
Rpim	0.109	0.026	0.817
Number of reflections	24543	783	3609
<I/σ(I)>	4
Completeness [%]	97.3	91.9	98.4
Redundancy	3	2.8	3.1
CC(1/2)	0.990	0.995	0.426

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.2	293	50 mM MES pH6.2, 10% PEG 550MME, 5% v/v glycerol and 50 mM CaCl2