6R0S
Cereblon isoform 4 from Magnetospirillum gryphiswaldense in complex with compound 4a and hydrolysis product
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-14 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 56.455, 58.824, 88.234 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.120 - 1.550 |
R-factor | 0.18815 |
Rwork | 0.187 |
R-free | 0.21293 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4v2y |
RMSD bond length | 0.012 |
RMSD bond angle | 1.780 |
Data reduction software | XDS |
Data scaling software | XDS |
Refinement software | REFMAC (5.8.0189) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.120 | 1.640 |
High resolution limit [Å] | 1.550 | 1.550 |
Rmerge | 0.090 | 0.980 |
Number of reflections | 43510 | 6861 |
<I/σ(I)> | 15.09 | |
Completeness [%] | 99.8 | 98.9 |
Redundancy | 12.66 | |
CC(1/2) | 0.999 | 0.850 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 294 | 0.4 M Ammonium phosphate |