6QZW
DPP8 bound to a dipeptide (MP) from the N-terminus of BRCA2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-09-12 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.00000 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 162.730, 246.074, 261.812 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 44.580 - 3.200 |
R-factor | 0.18599 |
Rwork | 0.184 |
R-free | 0.22478 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6eoo |
RMSD bond length | 0.003 |
RMSD bond angle | 0.984 |
Data reduction software | XDS |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0232) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 44.580 | 3.280 |
High resolution limit [Å] | 3.200 | 3.200 |
Rmerge | 0.134 | 1.310 |
Number of reflections | 86559 | 6331 |
<I/σ(I)> | 17.2 | 1.8 |
Completeness [%] | 99.9 | |
Redundancy | 8.37 | |
CC(1/2) | 0.990 | 0.730 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.75 | 277 | 10 mg/ml of protein, 0.46 M Na citrate. Drops are set in a 1:1 ratio |