6QZS
14-3-3 sigma in complex with FOXO1 pS256 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PETRA III, DESY BEAMLINE P11 |
Synchrotron site | PETRA III, DESY |
Beamline | P11 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-13 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.0332 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 63.429, 104.619, 156.866 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 58.800 - 1.900 |
Rwork | 0.182 |
R-free | 0.21580 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3mhr |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0230) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 58.800 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.105 | 0.847 |
Rmeas | 0.113 | 0.771 |
Number of reflections | 83148 | 4011 |
<I/σ(I)> | 13.02 | 1.71 |
Completeness [%] | 100.0 | 97.2 |
Redundancy | 12.7 | 10.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277.15 | 0.1 M Bis-Tris propane, pH7 0.2 M Sodium citrate tribasic dihydrate 20 % w/v PEG 3350 10 % w/v Glycerol |