6QYX
p38(alpha) MAP kinase with the activation loop of ERK2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-2 |
Synchrotron site | ESRF |
Beamline | ID23-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2015-09-05 |
Detector | DECTRIS PILATUS3 2M |
Wavelength(s) | 0.873 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 68.860, 74.500, 75.280 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 52.950 - 1.660 |
R-factor | 0.18774 |
Rwork | 0.185 |
R-free | 0.24233 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | p38 wild type |
RMSD bond length | 0.017 |
RMSD bond angle | 1.804 |
Data reduction software | MOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0218) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 52.950 | 1.720 |
High resolution limit [Å] | 1.660 | 1.660 |
Number of reflections | 46102 | |
<I/σ(I)> | 9.8 | |
Completeness [%] | 99.1 | |
Redundancy | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1 M HEPES pH 7.5, 0.2 M KF, 13%-17% (w/v) PEG 3350, 25 mM beta-D-octyl glucoside (bOG) |