6QVO

Crystal structure of human MTH1 in complex with N6-methyl-dAMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	Diamond
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-02-21
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.07
Spacegroup name	P 1 21 1
Unit cell lengths	35.478, 67.111, 117.678
Unit cell angles	90.00, 94.74, 90.00

Refinement procedure

Resolution	58.600 - 2.450
R-factor	0.23999
Rwork	0.238
R-free	0.28550
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	1.281
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	58.600	2.550
High resolution limit [Å]	2.450	2.450
Number of reflections	20436
<I/σ(I)>	3.2
Completeness [%]	100.0
Redundancy	6.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		293	0.1 M sodium acetate pH 3.5, 26 % PEG3350, 0.2 M LiSO4