6QVG
Human SHMT2 in complex with lometrexol
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-26 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.07 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 158.807, 158.807, 208.257 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 208.260 - 2.320 |
| R-factor | 0.20112 |
| Rwork | 0.199 |
| R-free | 0.23580 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.430 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 208.300 | 208.300 |
| High resolution limit [Å] | 2.320 | 2.320 |
| Number of reflections | 2640873 | |
| <I/σ(I)> | 14.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 39.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 291 | 0.1 M Bis-tris pH 6.5, 28 % PEGMME2000 |
