6QL9

Structure of Fatty acid synthase complex from Saccharomyces cerevisiae at 2.9 Angstrom

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-15
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9794
Spacegroup name	P 1 21 1
Unit cell lengths	217.616, 347.596, 265.266
Unit cell angles	90.00, 107.88, 90.00

Refinement procedure

Resolution	191.500 - 2.820
R-factor	0.19342
Rwork	0.192
R-free	0.21135
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2uv8
RMSD bond length	0.008
RMSD bond angle	1.513
Data reduction software	XDS
Data scaling software	Aimless (0.7.3)
Phasing software	MOLREP (11.6.04)
Refinement software	REFMAC (5.8.0238)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	191.500	2.900
High resolution limit [Å]	2.820	2.820
Rpim	0.073	1.096
Number of reflections	894158	4914
<I/σ(I)>	10.91	0.65
Completeness [%]	96.5	76.5
Redundancy	7.2	7.2
CC(1/2)	0.996	0.241

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1 M hepes pH 7.0, 0.3 M sodium-malonate pH 7.0 , 11.5-13% (w/v) PEG3350