6QH6

AP2 clathrin adaptor core with two cargo peptides in open+ conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-09-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.252
Spacegroup name	P 41 21 2
Unit cell lengths	180.500, 180.500, 210.060
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	91.000 - 5.000
R-factor	0.26043
Rwork	0.259
R-free	0.28519
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.525
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0189)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	91.000	5.590
High resolution limit [Å]	5.000	5.000
Rmerge	0.202
Rmeas	0.247
Rpim	0.138
Number of reflections	15525
<I/σ(I)>	3.6
Completeness [%]	99.6
Redundancy	4.8
CC(1/2)	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	289	7.5% PVA, 9% 1-propanol, 90 mM Hepes pH 7.4, 100 mM guanidine hydrochloride