6QDJ

Molecular features of the UNC-45 chaperone critical for binding and folding muscle myosin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P13 (MX1)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P13 (MX1)
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-12-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9763
Spacegroup name	P 1 21 1
Unit cell lengths	54.110, 111.960, 84.740
Unit cell angles	90.00, 97.44, 90.00

Refinement procedure

Resolution	84.026 - 1.884
R-factor	0.186
Rwork	0.184
R-free	0.21280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5m05
RMSD bond length	0.012
RMSD bond angle	1.057
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX ((1.13_2998: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.000	2.050
High resolution limit [Å]	1.884	1.884
Rmeas	0.045	0.454
Number of reflections	76208
<I/σ(I)>	19.6	2.9
Completeness [%]	93.8	78
Redundancy	3.3	2.9
CC(1/2)	0.999	0.866

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	100 mM MES/imidazole pH 6.5 10 % PEG 4000 20 % glycerol 20 mM 1,6-hexandiol 20 mM 1-butanol 20 mM (RS)-1,2-propanediol 20 mM 2-propanol 20 mM 1,4-butanediol 20 mM 1,3-propandiol